Establishment of a
reborn MMV-microarray technology: realization of microbiome analysis and other
hitherto inaccessible technologies
Harshita Sharma, Yasunori Kinoshita, Seiichi
Fujiu, Shota Nomura, Mizuho Sawada,Shamim Ahmed, Masaki
Shibuya, Kosaku Shirai, Syota Takamatsu, Tsuyoshi
Watanabe,Hitoshi Yamazaki, Ryohei Kamiyama, Tetsuya
Kobayashi, Hidenao Arai, Miho Suzuki,Naoto
Nemoto, Ki Ando, Hidekazu Uchida, Koichiro
Kitamura, Osamu Takei and Koichi Nishigaki
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BMC Biotechnology 2014, 14:78 doi:10.1186/1472-6750-14-78
Published: 21 August 2014
Background
With the accelerating development of bioscience, the problem
of research cost has become important. We previously devised and developed a
novel concept microarray with manageable volumes (MMV) using a soft gel. It
demonstrated the great potential of the MMV technology with the examples of
1024-parallel-cell culture and PCR experiments. However, its full potential
failed to be expressed, owing to the nature of the material used for the MMV
chip.
Results
In the present study, by developing plastic-based MMVs and
associated technologies, we introduced novel technologies such as C2D2P (in
which the cells in each well are converted from DNA to protein in
1024-parallel), NGS-non-dependent microbiome analysis, and other powerful
applications.
Conclusions
The reborn MMV-microarray technology has proven to be highly
efficient and cost-effective (with approximately 100-fold cost reduction) and
enables us to realize hitherto unattainable technologies.
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