Evaluation of Physarum polycephalum plasmodial
growth and lipid production using rice bran as a carbon source
- *Corresponding
author: Hanh Tran ttmhanh@hcmiu.edu.vn
1School of Biotechnology, Ho Chi Minh
International University, Ho Chi Minh City 70000, Vietnam
2Department of Biological Sciences, University of
Arkansas, Fayetteville 72701, Arkansas, USA
3Stable Isotope Laboratory, University of
Arkansas, Fayetteville 72701, Arkansas, USA
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BMC Biotechnology 2015, 15:67 doi:10.1186/s12896-015-0188-y
The electronic version of this article is the complete one
and can be found online at:http://www.biomedcentral.com/1472-6750/15/67
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Received:
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30 January 2015
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Accepted:
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24 July 2015
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Published:
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1 August 2015
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© 2015 Tran et al.
This is an Open Access article distributed under the terms
of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0),
which permits unrestricted use, distribution, and reproduction in any medium,
provided the original work is properly credited. The Creative Commons Public
Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/)
applies to the data made available in this article, unless otherwise stated.
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Background
The myxomycete Physarum polycephalum appears
to have remarkable potential as a lipid source for biodiesel production. The
present study evaluated the use of rice bran as a carbon source and determined
the medium components for optimum growth and lipid production for this
organism.
Results
Optimization of medium components by response surface
methodology showed that rice bran and yeast extract had significant influences
on lipid and biomass production. The optimum medium consisted of 37.5 g/L
rice bran, 0.79 g/L yeast extract and 12.5 g/L agar, and this yielded
7.5 g/L dry biomass and 0.9 g/L lipid after 5 days. The biomass
and lipid production profiles revealed that these parameters increased over
time and reached their maximum values (10.5 and 1.26 g/L, respectively)
after 7 days. Physarum polycephalum growth decreased on
the spent medium but using the latter increased total biomass and lipid
concentrations to 14.3 and 1.72 g/L, respectively.
Conclusions
An effective method for inoculum preparation was developed
for biomass and lipid production byP. polycephalum on a low-cost
medium using rice bran as the main carbon source. These results also
demonstrated the feasibility of scaling up and reusing the medium for
additional biomass and lipid production. TOMADO DE ENVIO DE BCM
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